theorchromo.ru

The BiolCCC system is an online tool developed by the Laboratory of Physical and Chemical Methods for Structure Analysis at the Institute for Energy Problems of Chemical Physics. It is designed to predict peptide/protein retention time in liquid chromatography based on the Liquid Chromatography of Biomacromolecules at Critical Conditions (BiolCCC) model. Users can access the system through the online version 1.0, where they can find help and support resources, including a mailing group and documentation. The system utilizes the Pyteomics library and offers access to the BiolCCC source code repository. To use the system, users need to describe their LC system by providing information such as column length, column internal diameter, packing material pore size, initial and final concentrations of component B, gradient time, delay time, flow rate, ACN concentration in component A, ACN concentration in component B, and the solid/mobile phase combination (RP/ACN+TFA or RP/ACN+FA). Once the system parameters are set, users can enter peptides, with each peptide listed on a new line. Cysteines in the peptides are carboxyamidomethylated according to the BiolCCC model. Overall, the BiolCCC system serves as a practical tool for researchers and scientists involved in liquid chromatography analysis. Its accurate prediction of peptide/protein retention time can facilitate chromatographic separations and contribute to advancements in the field of biomacromolecule analysis.